Excellent Phospho-p38 Antibody

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p38 Antibody

Catalog Number:

4511S

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse whole cell lysates

Primary Incubation

1:1000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X PBS

Secondary Incubation

1:2500 at room temperature for 2 hour

Tertiary Incubation

NA

Detection

Western Blot

Results Summary

High level of p38 phosphorylation was observed with stimulation with mycobacterial antigen.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Highly specific with no background

The Bad

None

The Bottom Line

Good antibody, with no background. Easy to Cell signal Technology data sheet for incubation and buffer instructions.

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