Nice CD19 Antibody

January 09, 2017

Microbiology
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Pacific Blue™ anti-mouse CD19 Antibody

Catalog Number:

115526

Browse Similar Products: CD19 Flow Cytometry Antibodies

We study virus-host interactions using a mouse model of infection. Here we were interested in what happens to B cell populations, specifically peritoneal B cells, following infection. Biolegend is our go-to company for flow cytometry reagents, and this CD5 antibody works great to distinguish B cells from other peritoneal cell types.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse peritoneal exudate cells

Primary Incubation

1:400 for 25 min at 4C

Blocking Agent

Fc blocking antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Good antibody for immunophenotyping B cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use

The Bad

N/A

The Bottom Line

Nice antibody. Easy to use. Little background signal.

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