Promega
Caspase-Glo® 3/7 Assay
g8090
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Aim: the goal of this experiment is to quantify the caspase 3/7 activity of mouse macrophages infected with viruses.Materials: infected or uninfected cells, pan-caspase inhibitor zVAD, and caspase-glo reagents.Method: follow the manufacture's protocol.Results: virus infection induces caspase 3/7 activity, which is inhibited by zVAD treatment.
Quantification of apoptotic cell death
Cell lysate from mouse macophages
Mouse macrophages were seeded in 24-well plate and infected with virus. After 18 hours infection, medium was removed and 120ul caspase-glo reagent/medium mixture was added to lyse the infected cells at RT for 20min. Follow manufacture's protocol for subsequent steps.
To save reagents, reducing the recommended amount of reagent won't affect the results relatively
In uninfected cells, the caspase 3/7 activity is low. Both virus 1 and virus 2 infection induce caspase 3/7 activity, but virus 2 is more potent. And caspase 3/7 activity is inhibited by zVAD.
N/A
Easy to use; low variation
Not found
A good way to quantify caspase 3/7 as an indication of apoptosis. Manufacture's protocol can be modified but won't affect the reliability of result.
Product Name: Caspase-Glo 3/7 AssaySupplier Page