Cytokine Multiplex Assays

January 19, 2017

University of Missouri
Vet. pathobiology
scientist

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Company:

Invitrogen

Product Name:

Cytokine Mouse Magnetic 20-Plex Panel

Catalog Number:

LMC0006M

To quantifying mouse cytokines, plasma was collected centrifuge at 400g for 15min and then stored at -80° C for batch analysis. Immune markers were evaluated in undiluted plasma using mouse specific multiplex bead-based, ELISA assay.

Experimental Design and Results Summary

Application

Assay Kits

Starting Material

Mouse blood

Protocol Overview

Plasma were collected and diluted before assay performance. Immune markers were evaluated using mouse specific multiplex bead-based, ELISA assay, Cytokine Mouse Magnetic 20-Plex Panel (LMC0006M) with the MAGPIX™ systems according to the manufacturer's instruction.

Tips

Time saving and and high throughput

Results Summary

Immune markers from target mouse were compared to control mouse using mouse specific multiplex bead-based, ELISA assay. 13 immune markers were evaluated to detect differences between two mouse groups.

DOI or PMID #

Preparing

Additional Notes

Easy-to-use protocols allow you to get your results in as little as 3.5 hours.

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Summary

The Good

Accurate, reproducible, and sensitive quantitation of multiple proteins

The Bad

Expensive

The Bottom Line

Accurate, reproducible, and sensitive quantitation of multiple proteins. Perform your multiplex assay and analyze your data typically in less than one day

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