Abcam
Mouse monoclonal (2G10) to beta-III-tubulin
ab78078
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This antibody did a good job as a dendrite marker for immunostaining, but was not usable for our assays due to the high background staining that appeared at speckles across the plate.
Immunofluorescence
Primary Mouse Neurons
1:750 in 5% Donkey Serum overnight at 4C
10% Donkey Serum at RT 1hr
1:400 Dylight donkey anti-mouse
None
Fluorescent Microscope
Antibody had a strong signal for the dendrites, but had background speckled reactivity that resulted in us not being able to use it for any automated analysis. We tried titrating the primary dilution down, but this did not help. We have not tried it on non-mouse samples which might be the problem.
N/A
Strong dendrite signal
Strong background speckling
Good antibody, but not in our samples due to the background issue.