Vector Laboratories, Inc.
Streptavidin/Biotin Blocking Kit
SP-2002
Info: View Product View Product Specs
Generally, we don't see to much background caused by biotin/streptavidin except for specific tissue samples, so we never had a need for a blocking kit for those. We recently took on a project that has a lot of background sources (mouse-on-mouse, biotin/streptavidin, peroxidase), so it was necessary to look for a blocking kit. In addition, we were asked by an investigator to replicate results from a previous protocol using a similar blocking kit. We would normally prefer to use polymer-based detection methods (such as with a Leica Bond RX), but we went with ABC-based method to match the previous protocol. We went with Vector Labs because we get a lot of our biotinylated secondaries and other associated reagents from them and have always had great results. The same was true with SP-2002. It was R.T.U. and required no additional tweaks. Just slide the steps in right before your primary antibody.
Immunohistochemistry
Mouse female reproductive tissue (specifically, cervix)
Deparaffinize with heat and xylenes. Rehydrate thru alcohol gradient (100%, 90%, 70%). Antigen retrieval. The Streptavidin/Biotin Blocking Kit. Protein Block. Primary antibody. Secondary Antibody. ABC Detection reagents. DAB. Hematoxylin. Coverslip
N/A
Cleared a lot of DAB brown tint from the background of our slides.
Price, Ease of Use
Product works as advertised. Price is on par or below competitors. No additional protocol tweaks necessary. Use with any Streptavidin/Biotin-based detection system.
Product Name: Streptavidin/Biotin Blocking KitSupplier Page