Anti-Human CD45RO For Memory T Cells

December 22, 2016

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

FITC Anti-Human CD45RO (UCHL1)

Catalog Number:

35-0457-T025

FITC Anti-Human CD45RO (UCHL1) was used in a sorting panel to separate memory and naive T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC from whole blood

Primary Incubation

PBMC were stained with an antibody cocktail containing CD45RO FITC (clone UCHL1) dilution 4/100 for 15min at RT at dark

Blocking Agent

FcR block (Miltenyi), 3min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Easy separation of memory and naive T cells in order to sort naive T cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice separation

The Bad

N/A

The Bottom Line

The separation looks normal and the memory and naive T cells are well separated and easy to gate.

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