Tonbo Biosciences
APC-Cy7 anti-human CD4 Antibody
25-0049
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Browse Similar Products: CD4 Flow Cytometry Antibodies
This antibody was used in a pediatric study involving healthy and asthmatic patients. Multiple phenotyping panels were used to screen patients for any changes in cell populations. T cells are part of one of these panels.This antibody works well to separate the CD3+ CD4+ T cells from CD3+ CD8+ T cells
Flow Cytometry
Human PBMC from whole blood
PBMC were stained with an antibody cocktail containing CD4 APC-Cy7 (clone RPA-T4) dilution 2.5/100 for 15min at RT at dark in total volume of 50ul
FcR block (Miltenyi), 3min
N/A
LSRII.
Nice separation of CD3+ CD4+ T cells from CD3+ CD8+ T cells.
Good separation, bright color
Donor dependent: the separation could smear a little