Lonza
Amaxa 4D Nucleofector X Unit
AAF-1001X
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After struggling with transfection of HUVECs using standard methods, the Lonza 4D nucleofection system was a godsend. Using the system I am able to to get high transaction efficiency with high viability. This is far superior to liposome based transfections
Transfection of siRNA/DNA
Live cells and basic cell culture buffers and media. P5 Primary Cell 4D-Nucleofector kit
Cells are trypsinised and, washed in PBS, spun down and resuspeneded in the transfection solution. Nucleotide is added (siRNA/DNA)
Work quickly, the less time the cells spend in transfection buffer the better. Have warm media ready. Cuvettes can be rinsed with H2O and reused.
High transfection efficiency with high viability
The system can transfect adherent cells (no need to trypsinize)
N/A
Great transfections
Each cell type requires a seperate kit to be purchased, can be expensive
Really great for difficult to transfect cells
Product Name: 4D-Nucleofector™ System, core unitSupplier Page