Invitrogen
Taq DNA Polymerase, recombinant
10342020
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Our lab has many genetically modified mouse lines and we need to use conventional PCR to genotype each liter of these animals. This kit makes it easy and gives us clean results.
PCR amplification for genotyping
DNA extracted from small mouse tail snips
DNA is extracted from small mouse tail snips (after digestion with Proteinase K) using a chloroform / ethanol protocol. Once DNA is isolated and in water, a master mix (containing PCR buffer, sterile water, MgCl2, primer mix, and Taq according to kit's protocol) is made and 19uL are added to 1uL of DNA. The samples are added to the thermocycler and the resulting samples are run on a 1.5% agarose gel and stained with ethidium bromide.
It's better to titrate the correct amount of MgCl2 needed.
We are able to amplify and clearly visualize our genes of interest using this PCR kit.
N/A
Efficient kit
Be careful of contamination and freeze/thaw cycles
This is a good kit for running a standard PCR reaction using isolated genomic DNA.
Product Name: Taq DNA Polymerase, recombinantSupplier Page