Biolegend
Purified anti-mouse CD45 Antibody
103102
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Browse Similar Products: CD45 Immunofluorescence Antibodies
Our lab uses this antibody to visualize CD45+ cells around the vascular structure in the mouse retina using whole mount staining.
Immunofluorescence
Fixed whole mouse retina
1:150 primary antibody, 2 hours at room temperature in staining buffer (or over night at 4 degrees Celsius)
Goat serum
1:250 primary antibody, 2 hours at room temperature in staining buffer (or over night at 4 degrees Celsius) - alexa 594
None
Fluorescent microscope
This antibody allowed us to visualize all the CD45+ cells on a whole mounted mouse retina and see their spatial relationship to the vasculature.
N/A
Works well
This antibody works well for marking CD45+ cells for immunofluorescence.