Ebioscience
Anti-Mouse IFN gamma PE
12-7311-82
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Browse Similar Products: IFN gamma Flow Cytometry Antibodies
The goal of this study was to determine whether different treatments affected B cell IFN gamma production by flow cytometry.
Flow Cytometry
Mouse Splenocytes
After isolating splenocytes, wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice.
Fc block
Wash cells once in FACS buffer. Stain with mouse CD19 antibody (1:500 dilution, 100 uL per million cells) for 20 minutes on ice.
Wash cells once in FACS buffer. Permeabilize cells at room temperature for 1 hour or overnight. Wash cells in permeabilization buffer Stain with isotype control or mouse IFNy antibody (1:100 dilution, 100 uL per million cells) for 20 minutes at room temperature in the dark.
Flow Cytometer
Relative to isotype control staining (left panel and empty histogram in right panel), IFNy-producing cells were readily visualized using this antibody (middle panel and shaded histogram in right panel).
N/A
Brightly stains IFN gamma.
No complaints.
Great antibody to distinguish IFN gamma-producing splenocytes.