Biolegend
Alexa Fluor® 488 anti-mouse CD31
102514
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We are seeking to understand the role of kidney resident macrophages in kidney inflammation. As a first step, we wanted to investigate the location of kidney macrophages in relation to endothelial cells and were we looking for a good antibody to stain endothelial cells. After testing many anti-CD31 antibodies, we selected this product due to the reliable and reproducible results we got.
Immunofluorescence
PLP fixed mouse (C57BL/6) kidney cryosections
Alexa Fluor® 488 anti-mouse CD31 (5ug/ml) for 3h at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
None
Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.
20um cryosections were stained with anti-CD31 Alexa Fluor® 488 (green) and counterstained with DAPI (blue). Nice staining of blood vessels.
27477514
N/A
Reliable, reproducible results
Nothing
Good antibody for IF