Effect of Proinflammatory cytokine on phosphorylation of P65 in HEK293 cells

NFkB-p65 is a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. Optimal induction of NF-kappaB target genes also requires phosphorylation of NF-kappaB proteins, such as p65, within their transactivation domain by a variety of kinases in response to distinct stimuli. The present study is aims to check the effect of proinflamatory cytokine on phosphorylation of P65 in HEK293 cells.