Expression of XIAP in HEK293 Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-XIAP Antibody

Catalog Number:

Sc-8789

X-linked inhibitor of apoptosis protein (XIAP) is a member of Inhibitor of apoptosis family; also known as inhibitor of apoptosis protein 3 (IAP3) and baculoviral IAP repeat-containing protein 4 (BIRC4). XIAP is a protein that stops apoptotic cell death by binding and inhibiting Caspases 3, 7 and 9. The present study aims to check the expression of XIAP in HEK293 cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293 cells

Primary Incubation

1:1000

Blocking Agent

No blocking

Secondary Incubation

1:5000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody (sc-8789) detected single band at around 55 kDA in HEK293 cells.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Nothing

The Bottom Line

Best antibody to detect XIAP, which produced single clear band by western blot

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