Anti-HA Magnetic Beads

Pharmacology
Boston University School of Medicine
Senior Postdoc Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific Pierce Protein Research Products

Product Name:

Pierce Anti-HA Magnetic Beads, 1mL

Catalog Number:

88836

HA is a tag commonly used in protein biochemistry. This product conjugates the anti-HA antibody to the magnetic beads allowing researchers to purify the HA-tagged proteins in short incubation.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

HEK293T and HeLa cell lysates expressing HA-tagged protein

Primary Incubation

Follow the instruction comes with the beads

Blocking Agent

No need

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ECL and Bio-Rad ChemiDoc

Results Summary

Cells expressing HA-tagged protein were lysed in lysis buffers such as CSK or NETN. With a short incubation of 15 to 20 mins at room temperature, this product efficiently immunoprecipitates HA proteins from human cell lysates.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Time saving and high sensitivity

The Bad

Longer incubation may result in higher background

The Bottom Line

The immunoprecipitation is very efficient and saves time. The price is fair and the bead-antibody can be recycled if needed.

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