Cell Signaling Technology
Phospho-Akt (Ser473) (D9E) XP Rabbit mAb
4060
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The general aim of my research is to study signaling pathways involved in the pathogenesis of leukemia by specifically studying signaling in acute myeloid leukemia (AML) stem cells. The specific goal of the experiment for which I used the product was to determine whether AML cells have active/phosphorylated Akt. The reason I selected this product is because I have had previous success with Cell Signaling phospho-antibodies.
Western Blot
HEL AML cell line
1:1000, overnight at 4 degrees C with rocking
TBS + 0.1% Tween + 5% w/v BSA
1:5000 anti-rabbit-HRP, 1 hour at room temperature with rocking
None
ECL
Clear band at the expected size (60 kDa) but also a band between 55-60 kDa and ~90 kDa.
N/A
Responds to cytokine stimulation
Unclear whether the antibody binds to other proteins aside from Akt as there are extra bands
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