IL-17A Antibody for Flow Cytometry

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse IL-17A Antibody

Catalog Number:

506908

I am interested in studying the immune system of the murine colonic lamina propria. Therefore, as a first step we had used a panel of antibodies to check the frequency of different types of immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

IL17A positive cells are clearly distinguished with this antibody

DOI or PMID #

N/A

Additional Notes

For IL17A staining, intracellular antigen staining protocol (eBioscience) was followed

Related Categories

Image Gallery

Summary

The Good

Very clear staining with no background problem

The Bad

None

The Bottom Line

Highly recommended!

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