CD8 antibody for Flow Cytometry

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD8a Antibody

Catalog Number:

100706

I am interested in studying the immune system of the murine colonic lamina propria. Therefore, as a first step we had used a panel of antibodies to check the frequency of different types of immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

CD8 positive cells are clearly distinguished with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very clear staining with no background problem

The Bad

None

The Bottom Line

Highly recommended!

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