BD Biosciences
BD Pharmingen™ PE-Cy™7 Mouse Anti-Human TNF
560923
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Browse Similar Products: TNF Flow Cytometry Antibodies
We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by activating them via TCR specific MHC-peptide ligands, and co-stimulatory molecules. We use purified CD8 T cells from naive donors with HLA types, such as B27 and A2, that have been described to mount potent anti-HIV responses to perform in vitro killing assays. The data shown here represents results from a positive control, where CD8 T cells were activated with PMA and ionomycin and then stained for degranulation markers, granzyme B, perforin, and different cytokine production.
Flow Cytometry
Purified CD8+ T Cells from human PBMC
1:50 for 30 min at 37 degrees followed by overnight incubation at 4 degrees Celcius
None
Flow cytometry
Nice staining
N/A
Works great.
I recommend it.