STEMCELL Technologies
STEMdiff Definitive Endoderm Kit
05110
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The aim of this experiment was to derive definitive endoderm in human iPS cells using the defined animal component-free medium.
Developmental biology-based studies
Human iPS or ES cells
The protocol was followed as per the manufacturer's guidelines.
You can use Rock Inhibitor and mTeSR media instead of the single cell passaging medium
Definitive endoderm is assessed by flow cytometry measurement of CXCR4 and cKIT. Both markers were highly expressed on the Day4.
N/A
Robust and easy to follow protocol.
Expensive
Easy to follow protocol. Reproducible generation of definitive endoderm.
Product Name: STEMdiff™ Definitive Endoderm KitSupplier Page