Enzo Life Sciences
HO-1 polyclonal antibody
ADI-SPA-895
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Browse Similar Products: HO-1 Immunohistochemistry Antibodies
This antibody was used to analyze the expression of HO-1 after a brain injury in the mouse. This antibody was recommended by a collaborator.
Immunohistochemistry
Mouse brain
1:100, overnight at 4 degrees Celsius, in blocking agent
10% goat serum, 0.2% TritonX100 in PBS (blocking 1.5h at room temperature)
1:500 biotinilated goat anti-rabbit (#BA1000, Vector lab.) in PBS containing 5% goat serum and 0.01% TritonX100, 1.5h at room temperature
30 min at room temperature in ABC kit (Vector lab.), then 5 min in PBS containing DAB and H2O2.
Bright-field microscope
Good antibody to detect HO-1 in mouse brain, it works well with DAB but also with fluorescence.
N/A
Also works well with fluorescence
Easy to optimize, nice staining
1:100 is a little high but worth it
Very good antibody to study the expression of HO-1 in the mouse brain.