Expression Of E2F1 In Human Aortic Smooth Muscle Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

Anti-E2F-1 Antibody

Catalog Number:

sc-251

E2F1 belong to a subclass of E2F factors, which acts as transcriptional activators essential for cell cycle regulation and progression. The present study aims to check the expression of E2F-1 in Human Aortic Smooth Muscle Cells by Western Blot. The above antibody identifies E2F1 at right size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle (HASM) Cells - Whole Cell Lysate

Primary Incubation

1:500

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

The Antibody (SC-251) detected E2F1 protein at right size (60 kDa) in Human Aortic Smooth Muscle Cells

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Higher Concentration of Primary Antibody Required

The Bottom Line

The Antibody (SC-251) detected E2F1 protein at right size (60 kDa).

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