Excellent Flow Cytometry Antibody For Mouse CD4

January 03, 2017

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

eBioscience

Product Name:

Anti-Mouse CD4 FITC

Catalog Number:

11-0041-81

I used to this antibody to stain CD4+ T-cells in mouse spleen, thymus and lymph nodes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:500 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I always get a nice distinct CD4+ population of T-cells which is of presentable quality.

DOI or PMID #

NA

Additional Notes

Works well before and after permeabilization

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Nothing

The Bottom Line

Best choice

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