Excellent Flow Cytometry Antibody For Mouse Foxp3

December 22, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

eBioscience

Product Name:

Anti-Mouse/Rat Foxp3 PE

Catalog Number:

12-5773-80

I used to this antibody to stain Foxp3+ T-cells in mouse spleen, thymus and lymph nodes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I always get a nice distinct Foxp3+ population of Treg-cells which is of presentable quality.

DOI or PMID #

NA

Additional Notes

I used Foxp3 transcription Fix/Permeabilation Kit from Tonbo Bioscience for fixation and permeabilization

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

Worth buying it

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