Shaking Incubator

National Institute of Nutrition
Biochemistry
Senior research fellow

Overall

Performance

Ease-of-Optimization

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Company:

New Brunswick Scientific

Product Name:

Benchtop Incubator Shaker

Catalog Number:

8261-30-1008

This Brunswick Incubator Shaker is useful for many laboratory applications like cell culturing, cell aeration, oxygen and equal distribution of nutrients. It can maintain the stable temperature conditions which is essential for culture growth. Uniform Shaking prevents bacterial biofilm formation, ensuring prolific bacterial reproduction.

Experimental Design and Results Summary

Application

Transformation of Escherichia coli with plasmids

Starting Material

Escherichia coli bacteria culture

Protocol Overview

E.Coli was isolated in pure form and transfected with plasmid. This bacterial culture was grown in a large scale in conical flask which contained selective media. This was incubated at 37°C for one day in this shaking incubator 200 rpm.

Tips

Check for the temperature and rotation maintenance.

Results Summary

At end of my experiment i found 50-60 % of bacterial cells got transformed with my plasmid.

Features Summary

It holds more space in the chamber and easy to handle.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to handle.

The Bad

It requires more room

The Bottom Line

Good to have such instrument in labs.

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