PE Mouse Anti-Human CD14 Clone M5E2 (RUO) lot 3241535

December 01, 2016

Biomedical Sciences
Cornell University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Pharmingen

Product Name:

PE Mouse Anti-Human CD14  Clone  M5E2  (RUO)

Catalog Number:

555398

HL-60 cells are able to differentiate to neutrophils after all trans retinoic acid (RA) treatment. After RA treatment they should be CD14 negative. HL-60 cells are also able to differentiate to monocytes after 1,25-dihydroxyvitamin D3 (D3) treatment. A characteristic of this conversion is CD14 expression. Cell culture volumes containing one million cells were spun down and resuspended in 200µl PBS with 2.5µl CD14 BD 555398 antibody. This antibody has high specificity and is at a competitive price compared to similar antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

HL-60 cells ; untreated, all trans retinoic acid (RA), 1,25-dihydroxyvitamin D3 (D3) treated

Primary Incubation

1 million cells were incubated 1h , 37C, in 200µl PBS with 2.5µl CD14 antibody

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Instrument: BD LSRII. PE was excited at 488 nm and emission was collected through 550 long-pass dichroic and 576/26 band-pass filters.

Results Summary

PE Mouse Anti-Human CD14 Clone M5E2 (RUO) has low background and good resolution. We used 2.5ul per test sample compared to 20ul recommended by BD.

DOI or PMID #

10.1371/journal.pone.0098929

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good specificity and resolution and has a competitive price compared to other similar antibodies ($0.34/sample).

The Bad

It is still expensive for what it is (an antibody).

The Bottom Line

The PE Mouse Anti-Human CD14 Clone M5E2 (RUO) BD 555398 antibody is a good antibody. The review is for lot 3241535.

Share your experience with other scientists. Write a Review! »

Join the discussion