Best Anti-Mouse CD62L Antibody For Flow Cytometry Analysis

December 07, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

eBioscience

Product Name:

Anti-Mouse CD62L (L-Selectin) PE-Cyanine7

Catalog Number:

25-0621-81

I used this antibody to phenotype naïve and memory T-cells based CD45low CD62Lhi and CD44hiCD62Llow expression pattern. I got nice distinct naïve and memory populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I always get a nice distinct naïve and memory populations of T-cells which are of presentable quality.

DOI or PMID #

NA

Additional Notes

Works with surface staining, but not with staining after permeabilization

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and reproducible results

The Bad

Little expensive

The Bottom Line

Worth buying it

Share your experience with other scientists. Write a Review! »

Join the discussion