Excellent Flow cytometry antibody for EpCAM

December 02, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD326 (EpCAM) PE-Cyanine7

Catalog Number:

25-5791-80

I used this antibody to stain mouse thymic epithelial cells which characterized as CD45-EpCAM+. I got nice separated population upon staining with this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Thymocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I always get a nice distinct EpCAM+ population of T-cells which is of presentable quality.

DOI or PMID #

NA

Additional Notes

Discount population was obtained after liberase digestion

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Little expensive

The Bottom Line

Worth buying it

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