Good Flow Cytometry Anti-Mouse/Rat Ki67 Antibody

December 01, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

eBioscience

Product Name:

Anti-Mouse/Rat Ki-67 PE

Catalog Number:

12-5698-80

I used this Ki67 antibody to stain proliferating T-cells as Ki67 is widely accepted as a proliferation marker.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I got nice Ki67 staining with proliferating T-cells which was further confirmed by simultaneous Cell trace staining as well.

DOI or PMID #

NA

Additional Notes

I used Foxp3 transcription Fix/Permeabilation Kit from Tonbo Bioscience for fixation and permeabilization

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Little expensive

The Bottom Line

Worth buying it

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