CD14PE-Tonbo 50-0149

December 05, 2016

Biomedical Sciences
Cornell University
Research Associate

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Company:

Tonbo Biosciences

Product Name:

PE Anti-Human CD14 (61D3)

Catalog Number:

50-0149

HL-60 cells are able to differentiate to neutrophils after all trans retinoic acid (RA) treatment. After RA treatment they should be CD14 negative. HL-60 are also able to differentiate to monocytes after 1,25-dihydroxyvitamin D3 (D3) treatment. A characteristic of this conversion is CD14 expression. Cell culture volumes containing one million cells were spun down and resuspended in 200µl PBS with 2.5µl CD14 Tonbo 50-0149 antibody. This antibody has high specificity and is at a competitive price compared to similar antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

HL-60 cells ; untreated, all trans retinoic acid (RA), 1,25-dihydroxyvitamin D3 (D3) treated

Primary Incubation

1 million cells were incubated 1h , 37C, in 200µl PBS with 2.5µl CD14 antibody

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Instrument: BD LSRII. PE was excited at 488 nm and emission was collected through 550 long-pass dichroic and 576/26 band-pass filters.

Results Summary

PE Mouse Anti-Human CD14 (61D3) has good resolution. We used 2.5ul per test sample compared to 5ul recommended by Tonbo.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Good specificity and resolution and has a competitive price compared to other similar antibodies ($0.705/sample). The Tonbo reps are among the friendliest reps and often give discounts.

The Bad

It is still expensive for what it is (an antibody) and has background a little higher than the BD CD14 PE.

The Bottom Line

The PE Mouse Anti-Human CD14 (61D3) Tonbo 50-0149 antibody is a good antibody. The review is for lot c0149120914502.

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