Expression of ORC4 in Human Aortic Smooth Muscle Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

Anti-ORC4 Antibody

Catalog Number:

sc-136331

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. ORC4 is component of ORC complex. The aim of the study is to detect ORC4 in Human aortic smooth muscle cells by western blot. The antibody (sc-136331) used here detected ORC4 at 45 kDA in Human Aortic Smooth Muscle Cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle (HASM) Cells - Whole Cell Lysate

Primary Incubation

1:200

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

The antibody used in the present study detected ORC4 at right size in Human Aortic Smooth Muscle Cells by western blot.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Higher Concentration of Primary Antibody Required

The Bottom Line

Antibody detected ORC4 in Human Aortic Smooth Muscle Cells by western blot.

Share your experience with other scientists. Write a Review! »

Join the discussion