Expression of MCM7 in Human Aortic Smooth Muscle Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-MCM7 Antibody

Catalog Number:

sc-9966

MCM7 - mini-chromosomal maintenance protein 7, which is a component of hexameric MCM2-7 complex (MCM complex). This is essential for DNA replication initiation and elongation in eukaryotic cells. The aim of present study is to detect the MCM7 in Human Aortic Smooth Muscle Cells by western blot. The antibody (sc-9966) used detected the right MCM7 protein at right size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle (HASM) Cells - Whole Cell Lysate

Primary Incubation

1:500

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

The Antibody used in the study detected MCM7 at around 88 kDa by western blot.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Nothing

The Bottom Line

Antibody detected the right MCM7 at right size at around 88 kDa.

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