BD Biosciences
BV421 Mouse Anti-Human CD11c Clone 3.9 (RUO)
565806
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Browse Similar Products: CD11c Immunofluorescence Antibodies
The overall goal of my research is to study HIV transmission in receptive anal intercourse. The specific goal is to study the HIV target cell abundance in different tissues. This antibody was chosen as company site indicates that it was previously successfully tested in Rhesus Macaques and company also states that it was tried in fluorescence applications, this color is commonly used for flow applications.
Immunofluorescence
Rhesus macaque tissue
One hour at room temperature
PIPES/Formaldehyde
None
Microscopy
Antibody worked great! Very nice and specific signal was detected with minimal background was detected in the blue channel since antibody is so bright. The only downside is that it is not recommended to use DAPI or other nuclei staining dyes as this color is in blue too. For that reason I chose to stain nuclei with DRAQ5 which is in far red channel.
N/A
Easy one our incubation, very bright
Great antibody for identification of macrophages and dendritic cells in Rhesus Macaque Tissue via fluorescent microscopy.