Thermo Fisher Scientific
SYTO 9 Green Fluorescent Nucleic Acid Stain
S34854
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This is a green fluorescent nucleic acid stain kit, shown to stain live and dead Gram-positive and Gram-negative bacteria. Overall, the product is OK when you have a clean pool of bacteria, works fine with Gram+ and Gram- as described. No significant quenching problem, very nice. Stains both DNA and RNA, works with both live and dead cells. It is a component of the LIVE/DEAD BacLight Bacterial Viability Kits. Problems I encounter: 1, not all cells get stained, not sure the reason, but some bacteria just have no color. 2, when the bacteria pool is dirty, does not work well, no much stain can be seen overall. 3, expensive.
Fluorescent Nucleic Acid Stain
Live/dead bacterial
Have a pool of bacteria; add SYTO 9 green fluorescent nucleic acid stain 50 nM–20 µM; Vortex to mix, then incubate for 1–30 minutes; visualize by microscope
Do not use excess dyes, titration is required
This dye stains both DNA and RNA, works with both live and dead cells. My cells are live bacteria cells, most of them get stained with the dye, but not every single cell.
N/A
Easy to use; standard protocol; no quenching
Not all cells get stained; expensive
Works with Scope and FACS
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