R&D Systems
Mouse PDGF R beta Antibody
AF1042
Info: View Product View Product Specs
Browse Similar Products: PDGF R beta Immunofluorescence Antibodies
The aim is to stain mouse skin pericytes. This antibody seems to work. However, the other cells in the skin also were stained. If you are looking for an antibody just for pericytes in mouse skin, it may not be the good choice. Anti-desmin is probably a better candidate.
Immunofluorescence
Mouse skin
4C overnight
Serum
45 min, RT, Rabbit anti-goat Alexa fluor 488
None
Alexa fluor 488
We used CD31 (stained in red) as the endothelial cell marker. The orange stained cells are double positive for CD31 and pericytes. However, you can see green stained cells in the other parts of the skin. So PDGFRb in the skin is not specific for pericytes. It may be expressed by other cells such as smooth muscle cells, myofibroblasts and mesenchymal stem cells.
N/A
Works
Not exclusively for skin pericytes
It works in mouse skin, but it is probably not a good choice for exclusive skin pericyte staining.