BD Biosciences
BV650 anti-mouse CD62L
564108
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This review is about L-selectin staining on mouse lymphocytes. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T lymphocytes, neutrophils, monocytes, and eosinophils. L-selectin is rapidly shed from lymphocytes and neutrophils upon cellular activation, which is what I wanted to test. It can also be used for differentiating effector vs central memory T cells within CD44high subset.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on BV650 channel
The staining showed that most cells were positive for CD62L. Within the activated cells, CD62L was down-regulated, which was expected and consistent with literature.
N/A
The staining was very bright and relevant
Wide range of expression; maybe an issue with compensation
Works well and gives optimal results