Biolegend
BV510 anti-mouse CD4
100559
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I used this antibody as a compensation control for a high bandwidth flow cytometry experiment. Although, it served the purpose i needed it for, it was very clear that I would not use this antibody for staining CD4 T cells in an assay. There was quite a bit of background and the staining was all over the place. Not worth it.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on BV510 channel
Although there was a positive staining, the population of positive cells was not distinct but all over the place.
N/A
Easy to use
Background staining and lots of bleeding
Not recommended for CD4 specific stains, but its fine as a comp control.