Use Of ZO-1 Antibody From ThermoFisher Scientific On Myogenic Cell Culture

November 16, 2016

Pathology
BWH
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by ThermoFisher Scientific

Product Name:

ZO-1 Polyclonal Antibody

Catalog Number:

61-7300

This antibody was used to analyze the distribution of ZO-1 in embryonic tissues. This polyclonal anti-ZO-1 antibody appears highly specific to tight junctions in epithelial tissues.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Chicken embryonic epithelial tissue

Primary Incubation

1:150, overnight, at 4C in Blocking agent, on slide

Blocking Agent

TBS, 1% FBS, 0.1% TritonX100, 30min, at RT

Secondary Incubation

1:500, AlexaFluor-488-conjugated anti-rabbit IgG secondary (Molecular Probes), overnight, at 4C in Blocking agent, on slide

Tertiary Incubation

N/A

Detection

Fluorescent microscope, FITC/GFP filter for AlexaFluor-488 detection

Results Summary

This antibody works well on fixed adherent embryonic tissue. Specificity for epithelia, at the apical level.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific signal at tight junction in epithelia by immunofluorescence

The Bad

Signal is moderately strong

The Bottom Line

Good antibody overall. Recommended for immunofluorescence study of epithelia. Works both on chicken and mouse tissue. This antibody reacts against Zonula-occludens protein 1 at the level of tight junctions.

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