PE Mouse Anti-Human CD11b/Mac-1 Clone ICRF44 (also known as 44) (RUO) lot 4314750

November 16, 2016

Biomedical Sciences
Cornell University
Research Associate

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Quality of Results

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human CD11b/Mac-1  Clone  ICRF44 (also known as 44)  (RUO)

Catalog Number:

555388

HL-60 cells are able to differentiate to neutrophils after all trans retinoic acid (RA) treatment. They are also able to differentiate to monocytes after 1,25-dihydroxyvitamin D3 (D3) treatment. A characteristic of this conversion is CD11b expression. Cell culture volumes containing one million cells (untreated vs. RA treated) were spun down and resuspended in 200µl PBS with 2.5µl CD11b 555388BD antibody. This antibody has high specificity and is at a competitive price compared to similar antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human HL-60 cells ; untreated, all trans retinoic acid (RA), 1,25-dihydroxyvitamin D3 (D3) treated

Primary Incubation

1 million cells were incubated 1h , 37C, in 200µl PBS with 2.5µl CD11b antibody

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Instrument BD LSRII. PE was excited at 488 nm and emission was collected through 550 long-pass dichroic and 576/26 band-pass filters.

Results Summary

PE Mouse Anti-Human CD11b/Mac-1 Clone ICRF44 (also known as 44) (RUO) has very low background and good resolution. We used 2.5ul per test compared to 20ul recommended by BD.

DOI or PMID #

N/A

Additional Notes

None

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Summary

The Good

Low background, good resolution, and has a competitive price compared to other similar antibodies

The Bad

It is still expensive for what it is (an antibody)

The Bottom Line

The BD 555388 PE Mouse Anti-Human CD11b/Mac-1 Clone ICRF44 (also known as 44) (RUO) antibody is a good antibody. The review is for lot 4314750, but we used this catalog number for many years and the lots were consistent.

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