Expression of MCM6 in Human Aortic Smooth Muscle Cells

November 16, 2016

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-MCM6 Antibody

Catalog Number:

sc-9843

Mini-Chromosome maintenance (MCM) protein 6 (along with other MCM proteins as a complex) - regulators of DNA replication. Here the expression of MCM6 in Human aortic smooth muscle cell lysate was detected by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle Cells

Primary Incubation

1:500

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody detected MCM6 protein at around 105 kDA in whole cell lysate

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Produces little background

The Bottom Line

Good antibody to detect MCM6

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