Santa Cruz Biotechnology
Aldose Reductase Antibody (H-6)
sc-166918
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Aldose reductase is an rate limiting enzyme of polyol pathway which is more The aim of the experiment was to find out any changes in the levels of aldose reductase protein in the lens of diabetic rats 24 hours after the induction of diabetes. Specific aim of the experiment was to compare the levels of aldose reductase protein in the eye lens of diabetic and normal rats.This product was expected to detect specifically the aldose reductase protein in rat tissue. The sample western blot showed on the SantaCruz website made us more reliable on the product as the bands are more dominant and specific.
Western Blot
Rat lens soluble protein
Overnight
Skimmed milk powder
2 hours
Not applicable
Chemiluminiscence
Antibody gave specific signal for aldose reductase protein. However, there was slight non specific binding at higher protein loading.
Results are not published
None
Specific signal
Not good at higher protein concentrations
Can be used to detect very small quantities of protein in animal tissue sample.