Thermo Scientific
Supersignal west dura extended duration substrate
34076
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In the present study, low abundant pancreatic proteins such as cdc42 and c-Raf were detected in pancreatic whole tissue lysate prepared from diabetic and non-diabetic rats.
Chemiluminiscent detection of low abundant proteins in x-Ray film
Rat pancreatic whole tissue lysate, SDS-PAGE assembly, immunoblotting assembly, x-Ray films, autoradiography cassette, x-ray film developer and fixer etc.
Diabetic and non-diabetic rats were sacrificed following standard ethical conditions. Pancreatic tissues from rats were harvested and whole tissue extract were prepared in protein extraction buffer. Further, an equal amount (20 micro gram) of proteins were resolved in polyacrylamide gel electrophoresis and western transferred to PVDF membrane. Membrane were probed for cdc24 and c-Raf proteins and treated with HRP conjugated secondary antibody. Probed blots were ECL developed with Supersignal west dura extended duration substrate for 30 seconds and signals were captured in x-Ray film
No
Very low abundant proteins like cdc42 and c-raf were detected very easily detected in x-ray film. Supersignal west dura extended duration substrate gave signals of target proteins by exposing x-ray film only for 30 seconds.
Highly sensitive extended duration substrate which maintain signals up to 10 hrs
Excellent quality product from Thermo Scientific
Product Name: SuperSignal West Dura Extended Duration Substrate, 200mL kitSupplier Page