Bertin
Soft tissue homogenizing CK14 – 2 mL
KT03961-1-003.2
I use the homogenizing kits for the Precellys system from Bertin corporations. These are expensive and need to be operated with the special Precellys equipment. However, they do make lysates easy. All you have to do is place the tissue in the tubes, add buffer with inhibitors, and put into the machine. After centrifuging, you have good quality lysates. This is great for labs that have the money and want to cut down on time. My critique of these kits, however, is the packaging. Many times they arrive with one of the tubes busted! Once I even got one with a dead fruit fly in it! At several dollars per tube, I wish I could count on every tube being usable.
Western Blotting
Mouse brain tissue
Place the mouse tissue in the tube and freeze for later extraction. Extraction...add buffer at 5% of weight of tissue (T-PER with EDTA and protease and phosphatase inhibitors (1% of solution)). Homogenize at 2x30s@5000rpm with Precellys homogenizer. Centrifuge 12,000xg for 15 min at 4 degrees. Extract the supernatant as your lysate.
The tubes will only hold approximately 1.7mL after being filled with brain tissue. If your tissue is too much, your protein will be really concentrated and you can't add 5% solution. This is not a big deal though.
Good quality lysates that can be run on Westerns.
None
Ease of use
Price
Good if you have the money.