The Antibody Stains Splenic B220+ Cells Very Well

November 01, 2016

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by Thermo Fisher Scientific

Product Name:

CD45R Antibody, PE-Texas Red

Catalog Number:

RM2617

We wanted to study B220+ cells in the healthy vs disease state, so we used this antibody to gate on B220+ cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The B220+ cells in the spleen are stained very clearly with this antibody

Additional Notes

None

Related Categories

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Summary

The Good

The antibody stains B220+ cells well

The Bad

None to mention

The Bottom Line

The antibody distinguishes B220+ cells in the spleen very clearly

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