Anti-CD45 Identifies Immune Cells Very Well In The Lymph Nodes

October 31, 2016

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV395 Rat Anti-Mouse CD45

Catalog Number:

565967

We wanted to examine immune cells in the health vs disease state, and therefore, used this antibody to identify/gate on the immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse lymph node cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody distinguishes the CD45+ cells in the lymph nodes very well

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

The antibody stains CD45+ very clearly

The Bad

None to mention

The Bottom Line

The antibody is great for identifying immune cells

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