PE Mouse Anti-Human CD38 Clone HIT2 (RUO) cat 555460 lot 5027616

October 28, 2016

Biomedical Sciences
Cornell University
Research Associate

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human CD38  Clone  HIT2  (RUO)

Catalog Number:

555460

HL-60 cells are able to differentiate to neutrophils after all trans retinoic acid treatment. A characteristic of this conversion is CD38 expression. We treated HL-60 cells with all trans retinoic acid 0.1 µM final concentration for 48h. Cell culture volumes containing one million cells (untreated vs. RA treated) were spun down and resuspended in 200µl PBS with 2.5µl CD38 antibody. CD38 antibodies from three companies were compared. BD PE Mouse Anti-Human CD38 Clone HIT2 (RUO) has very low background, good resolution, and an affordable price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human HL-60 cells ; untreated, all trans retinoic acid (RA), 1,25-dihydroxyvitamin D3 (D3) treated

Primary Incubation

1 million cells were incubated 1h , 37C, in 200µl PBS with 2.5µl CD38 antibody

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Instrument BD LSRII. PE was excited at 488 nm and emission was collected through 550 long-pass dichroic and 576/26 band-pass filters.

Results Summary

BD PE Mouse Anti-Human CD38 Clone HIT2 (RUO) has very low background and good resolution.

Additional Notes

None

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Summary

The Good

BD PE Mouse Anti-Human CD38 Clone HIT2 (RUO) has very low background, good resolution and has a competitive price compared to other similar antibodies ($0.3/sample).

The Bad

Expensive. Although among the CD38 antibodies available on the market, this one is less expensive, it is still expensive for what it is.

The Bottom Line

The 555460 BD PE Mouse Anti-Human CD38 Clone HIT2 (RUO) antibody is a good antibody. The review is for lot 5027616, but we used this catalog number for many years and the lots were generally consistent.

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