RFP Staining in Mouse Lung

Cancer Centre
Cold Spring Harbor Laboratory
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Rockland

Product Name:

RFP Antibody Pre-adsorbed

Catalog Number:

600-401-379

RFP was used to stain lung nodules in our GEMM. We were then able to differentiate between cancerous and non-cancerous lung tissue.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse lung

Primary Incubation

RFP rockland in 0.5x buffer

Blocking Agent

1X blocking buffer made with 0.1%Triton X-100 in PBS

Secondary Incubation

Goat anti rabbit 568 in 0.5X blocking buffer

Tertiary Incubation

Vectastain

Detection

DAPI

Results Summary

RFP stained cancerous nodules in the lung and was selective. RFP worked and is publishable.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Publishable data

The Bad

N/A

The Bottom Line

RFP is great for detecting nodules in cancerous lung tissue.

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