Cell Signaling Technology
PCNA (D3H8P) XP Rabbit Antibody
13110S
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Browse Similar Products: PCNA Immunofluorescence Antibodies
Needed an antibody to detect PCNA to compare the S-phase population between different treatments. This one is good for immunofluorescence with a great specificity and sensitivity.
Immunofluorescence
Human U2OS cell line
1:800 in 2% BSA/PBS-0.05% Tween 20 for 2hr at room temperature
2% BSA/PBS-0.05% Tween 20
Anti-Rabbit-Cy3
1:300 of 2nd antibody at room temperature for 45min
Immunofluorescence microscope
This antibody works as good as the one (PC10) from Santa Cruz. The signal is specific and sensitive. I have tried it in not only U2OS, but also many other cell lines. The result is always reproducible. A good quality antibody that is highly recommended for detecting PCNA in mammalian cells.
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Great quality and sensitivity
N/A so far
With the consistent quality and reproducible results, this is definitely a great anti-PCNA antibody for many applications.