NK Cell Separation Beads

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD56 MicroBeads, human

Catalog Number:

130-050-401

Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of the capacity of effector cells, such as NK cells, to kill HIV-infected cell. We are currently investigating the capacity of an engineered antibody to trigger NK-cell mediated ADCC activity in in vitro assays against an HIV-infected cell line. We use purified human NK cells from donors to use as effector cells in these assays.

Experimental Design and Results Summary

Application

NK cell purification

Starting Material

Whole human PBMC

Protocol Overview

Use the kit's recommendations on the amount of beads to be added per certain number of cells. All their optimizations work really well.

Tips

Follow the kit instructions

Results Summary

Great purification of CD56+ cells

Additional Notes

None

Image Gallery

Summary

The Good

Very good cell separation

The Bad

None

The Bottom Line

I recommend its use.

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